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Radioprotective Effect of Laser Radiation With a Wavelength of 532 nm on Fibroblast Cells



Author(s)

Karine Voskanyan, Аnna Rzyanina, Gennady Mitsyn and Victor Gaevskiy

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DOI:10.17265/2159-5348/2018.02.003

Joint Institute for Nuclear Research, Dubna 141980, Russia

Studies have been carried out to test the assumption that the primary photoreceptors for the radioprotective action of 633 nm laser radiation are the cytochrome-c-oxidases. To do this, the device was created for radiation protection of biological objects based on laser module with a wavelength of 532 nm. Experiments conducted on murine fibroblast cells showed that the radioprotection effect of laser irradiation was observed in the dose interval about 0.4-0.85 mJ/cm². Maximum radioprotection effect is observed at laser radiation energy density ~ 0.56 mJ/cm². The determination of the cell survival with the automatic counter СТ20 after the action of the ionizing and combined irradiation showed that radioprotective action of the laser radiation with the wavelength of 533 nm, as well as the radiation with the wavelength of 633 nm, is transferred by the mechanism of the “bystander effect”. In addition, it was found that radioprotection effect of laser irradiation observed on the criterion of number of surviving single cells, compared with cells exposed to -radiation Laser irradiation, produces effective radioprotecting action also on the criterion of grown cell colonies. The value of the dose modification factor (DMF) calculated on 50% cell survival (LD₅₀) is equal to 1.4. The results suggest that in the case of radioprotective action of small doses of laser radiation with a wavelength of 633 nm, as well as 532 nm primary photoreceptors is the cytochrome-c-oxidases.

Gamma-radiation, laser radiation, radioprotective effect.